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ecis zθ instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis zθ instrument
    Ecis Zθ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+z%CE%B8+instrument/ECIS+Z+Theta/pm41227360-127-11-14
    Average 96 stars, based on 639 article reviews
    ecis zθ instrument - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Attachment Assay:

    Article Title: Fibulin‐4 and latent‐transforming growth factor beta‐binding protein‐4 interactions with syndecan‐2 and syndecan‐3 are required for elastogenesis
    Article Snippet: The dye was solubilized for 10 min in 10% acetic acid in TBS on a shaker at 500 rpm, and the absorbance was determined at 570 nm (Beckman Coulter, Model# DTX 880). .. To analyze cell attachment in real‐time, we used an Electric Cell‐Substrate Impedance Sensing (ECIS) Zθ instrument (Applied Biophysics) operated at 37°C at 5% CO 2 . .. The cells were analyzed in ECIS 96‐well plates (Applied Biophysics; #96W20idf PET).

    Electric Cell-substrate Impedance Sensing:

    Article Title: Fibulin‐4 and latent‐transforming growth factor beta‐binding protein‐4 interactions with syndecan‐2 and syndecan‐3 are required for elastogenesis
    Article Snippet: The dye was solubilized for 10 min in 10% acetic acid in TBS on a shaker at 500 rpm, and the absorbance was determined at 570 nm (Beckman Coulter, Model# DTX 880). .. To analyze cell attachment in real‐time, we used an Electric Cell‐Substrate Impedance Sensing (ECIS) Zθ instrument (Applied Biophysics) operated at 37°C at 5% CO 2 . .. The cells were analyzed in ECIS 96‐well plates (Applied Biophysics; #96W20idf PET).

    Article Title: Bezafibrate improves mitochondrial function, blood-brain barrier integrity, and social deficits in models of 22q11.2 deletion syndrome
    Article Snippet: .. Electric Cell-substrate Impedance Sensing (ECIS) methodology was employed making using of the ECIS ZΘ instrument and 8W10E+ electrode arrays (Applied Biophysics, Troy, NY, USA), and resistance was measured at 4000 Hz. ..

    Article Title: Acute Kidney Injury Induces Lung Damage via Mitochondrial DAMPs by Activating TREM-1 and cGAS-STING Pathways
    Article Snippet: .. The wounds were tracked in real-time at 4000 Hz using an ECIS Zθ instrument (Applied BioPhysics, Troy, NY, USA). .. 96W10idf ECIS Culturewares (Applied BioPhysics, Troy, NY, USA) were used for cell cytotoxicity assays as described elsewhere [ , ].

    Article Title: Acute Kidney Injury Induces Lung Damage via Mitochondrial DAMPs by Activating TREM-1 and cGAS-STING Pathways.
    Article Snippet: .. The resistance was recorded in real-time at 4000 Hz using an ECIS Zθ instrument (Applied BioPhysics, Troy, NY, USA). .. 8W1E ECIS Culturewares (Applied BioPhysics, Troy, NY, USA) were used for migration assays as described previously [30,33].

    Article Title: Acute Kidney Injury Induces Lung Damage via Mitochondrial DAMPs by Activating TREM-1 and cGAS-STING Pathways.
    Article Snippet: .. The wounds were tracked in real-time at 4000 Hz using an ECIS Zθ instrument (Applied BioPhysics, Troy, NY, USA). .. 96W10idf ECIS Culturewares (Applied BioPhysics, Troy, NY, USA) were used for cell cytotoxicity assays as described elsewhere [34,35].

    Article Title: EPLIN, a prospective oncogenic molecule with contribution to growth, migration and drug resistance in pancreatic cancer.
    Article Snippet: .. The plate was placed on the ECIS Zθ instrument (Applied Biophysics Ltd, Troy, New Jersey, USA) and incubated at 37 °C for 4–5 h, until a confluent monolayer was formed. ..

    Article Title: Natural Molecule-Derived Nanogels from Hematoxylin and l-lysine for Biomedical Use with Antimicrobial Properties
    Article Snippet: .. Resistances were recorded in real-time at 4000 Hz using an ECIS Zθ instrument (Applied BioPhysics, Troy, NY, USA). ..

    Article Title: Acute Kidney Injury Induces Lung Damage via Mitochondrial DAMPs by Activating TREM-1 and cGAS-STING Pathways
    Article Snippet: .. The resistance was recorded in real-time at 4000 Hz using an ECIS Zθ instrument (Applied BioPhysics, Troy, NY, USA). .. 8W1E ECIS Culturewares (Applied BioPhysics, Troy, NY, USA) were used for migration assays as described previously [ , ].

    Incubation:

    Article Title: EPLIN, a prospective oncogenic molecule with contribution to growth, migration and drug resistance in pancreatic cancer.
    Article Snippet: .. The plate was placed on the ECIS Zθ instrument (Applied Biophysics Ltd, Troy, New Jersey, USA) and incubated at 37 °C for 4–5 h, until a confluent monolayer was formed. ..



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    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
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    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
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    Image Search Results


    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing (ECIS). Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.

    Journal: bioRxiv

    Article Title: The role of aging on endothelial cell-cell junctions and pulmonary microvascular permeability

    doi: 10.1101/2025.07.29.667420

    Figure Lengend Snippet: Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing (ECIS). Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.

    Article Snippet: Cells were grown in complete DMEM, and resistance was continuously monitored at 4000 Hz with an electric cell-substrate impedance sensing (ECIS) instrument (Model Zθ, Applied Biophysics).

    Techniques: Permeability, Electric Cell-substrate Impedance Sensing, Staining, Avidin-Biotin Assay